Learner autonomy is the trait of an adult who displays agency concerning learning activities, whereas academic motivation is the primary driving force for students to learn, which is the drive and urge to achieve academically, while grit is an important personality trait for academic achievement. People with the motivation and persistence to work and study diligently despite hurdles and hardships to achieve a set of goals are more likely to be successful than those lacking these traits. The present study investigates the effect of learners' autonomy on academic motivation and university students' grit by employing a quantitative method. A survey method has conducted to collect the data from 1230 participants randomly selected from the general type universities. A theoretical framework has been proposed consisting of several components of learner autonomy, academic motivation, and grit. Learners' autonomy is based on social cognitive theory, while academic motivation and grit are based on self-determination theory. This study uses three instruments: Learner Autonomy Profile-Short Form (LAP-SF), academic motivation scale (AMS), and grit scale. The LAP-SF adapted consists of four constructs (desire, resourcefulness, initiative, and persistence), whereas the scale's reliability is 0.96. Similarly, the AMS developed by Vallerand adopted to collect data consists of three factors that have the reliability of a scale of 0.87. Lastly, data related to grit has been collected through the grit scale developed by Duckworth, which consists of two factors that have reliability of a scale of 0.81. The data has been analyzed through Linear Regression to answer the study research questions. Findings of the regression analysis with its four factors (Desire, Resourcefulness, Initiative, and persistence) revealed that LAS and AMS have a positive association whereas these factors also has a positive association between LAS and Grit Scale. It has been concluded that Learners 'Autonomy with all its sub-factors positively affects students' academic motivation and grit level.