The aim of this study was to adapt the Turkish version of the Questionnaire on Attitudes towards Disability in Higher Education (QAD-HE) developed by Fuentes, Pérez-Padilla, Fuente, and Aranda (2021) into the national literature by conducting validity and reliability studies. In this context, this study included findings related to the validity and reliability of the adapted scale. A total of 606 individuals from a state university participated in this study. Of the participants, 535 were students and 71 were teachers. The findings revealed that the Questionnaire on Attitudes toward Disability in Higher Education (QAD-HE), which was adapted within the scope of the research, contains 27 items and has a two-dimensional structure as "egalitarian attitudes" and "prejudiced attitudes." The findings showed that the Questionnaire on Attitudes toward Disability in Higher Education can be used to determine attitudes toward individuals with disabilities studying or working in higher education in Turkey.