In this study, it is aimed to reveal the role of the Big Five personalities in online learning attitude. In the research carried out with the relational screening model, 89 females ([X-bar] age=20.61±2.37) and 124 males ([X-bar] age=20.80±2.69) aged between 18 and 33, a total of 213 ([X-bar] age=20.71±2.57) sports science students studying in sports sciences faculties of three different state universities in Turkey participated. The data were collected using "Personal Information Form", "Online Learning Attitude Scale" and "Big Five-50 Personality Test". Pearson correlation and regression analyses were are in the statistical evaluation of the data. According to the research findings, there were positive and low level significant relationships between online learning attitude scores and extraversion, conscientiousness, and openness to experience scores. In addition, openness to experience was found to have a significant predictive power on online learning attitude. As a result; the role of the Big Five personalities in online learning attitude has been revealed in the current research.