This study aimed to identify the main stressors in the transition and adaptation processes to higher education in nursing students. Two reflective groups were conducted with 136 students. The thematic-reflexive analysis revealed that the main stressors were the excessive workload of course activities, the need for maturation, psychological pressure, and lack of financial resources. The results demonstrated the need for monitoring students throughout the course, especially in the early years, as well as the continuity of welcoming activities. University care programs can help adapt to the course, also functioning as mental health promoters.