Purpose: The purpose of this study is to determine whether the pandemic affects the motivation and job satisfaction of university researchers as a whole and by their separate groups by gender, age, academic position (career stage) and degree. Design/methodology/approach: The authors studied French universities for changes in motivation and job satisfaction among researchers under the impact of the COVID-19 pandemic. For this, two hypotheses were tested, using the one-way ANOVA parametric test and the Kruskal-Wallis nonparametric statistical test with the data collected during the in-depth interviews and surveys with university researchers from the Paris Diderot University and Jean Moulin University of Lyon III in 2019 and 2021. Findings: There were found significant differences between changes in motivation and job satisfaction of respondent groups by gender, career stage and scientific degrees. All these differences in changes confirm hypothesis about the significant impact of the pandemic on certain motivators and elements of job satisfaction of various groups of university researchers. The study showed that pandemic restrictions and fears positively affect the motivation and job satisfaction of the younger generation of researchers and males, and negatively on the older generation and female researchers. During the pandemic, early-stage researchers are mostly unhappy only with the salary and the lack of teaching skills. While experienced and senior researchers do not have time to fulfill all their duties due to overload by a combination of different forms of teaching, many teaching hours, administrative duties and mastering new communication tools. Research limitations/implications: The results capture the selective impact of pandemic restrictions, its physical and mental effects on academic staff. Although only two universities in a single country were considered, the findings show the heterogeneity of the perception of new (pandemic) working conditions by different groups of university workers. If such heterogeneity is revealed in the example of two universities, then it is very likely to be observable also in broader studies. Practical implications: The results of this study can help university administrations, higher education regulators and national research agencies address the needs of all groups of researchers, alleviating the most acute restrictions and fears caused by the pandemic and providing the necessary incentives for relevant groups, e.g. higher salary, teaching school and awards for young researchers and a lower teaching loading for experienced and senior researchers. Originality/value: This is the first study that define pandemic impact on motivation and job satisfaction of university researchers in France.