The call to increase student interest in science and related careers continues to receive national attention in the United States. While many current efforts have focused on infrastructure support and innovative pedagogies to better reach and engage students, research suggests that having a career role model can influence adolescents' interest in and choices of science courses and career pathways. This exploratory study investigated the impact of a week-long online biotechnology program called Biotech in Action (BIA) that featured career role modeling and authentic science learning. Students were engaged in career modeling sessions with multiple professionals working in related STEM fields while learning life sciences within authentic biotechnology research contexts. In total, over 400 high school students participated in BIA. The results showed that students felt they developed a better understanding of the biotechnology field and became more cognizant about steps to achieve their future career goals after BIA. Many students reported that the interactions with career role models and learning about their educational and professional pathways helped demystify science career fields. Overall, this research provides new insights to curriculum designers and researchers on integrating career role modeling and authentic learning to spark and sustain student interest in science.