This paper aims to identify the factors affecting the quality of the e-learning of physical education students. The statistical population of the paper consisted of undergraduate students at the University of Isfahan in the field of physical education who passed their theoretical and practical courses virtually in two semesters. The sample size was estimated to be 210 individuals based on the Krejcie and Morgan table. Then, a researcher-made questionnaire was employed to collect the required data. Exploratory factor analysis was used to identify the factors affecting the quality of e-learning and confirmatory factor analysis was used to confirm the identified factors. According to the results, the factors affecting the e-learning quality of theoretical and practical courses are teachers' characteristics, technical characteristics of the educational system, content, student characteristics, and evaluation and feedback.