The mindfulness-based methods are on the rise in the mental health care of students as well as employees. Therefore, the research on self-compassion is necessary to explore abilities and personality traits that are cultivated by the mindfulness approach. Our research deals with the assessment of the level of self-compassion by the students of the Faculty of Economics and Management at the Czech University of Life Sciences to precise the planned mindfulness-based intervention. Further, the gender and personality specifics as well as a connection to academic achievement are examined. For this purpose, the Self-compassion Scale, and the NEO-PI-R were used. The results proved insignificant correlations between the self-compassion subscales and self-reported grades, but also subtle differences in the structure of the self-compassion by males and females. Further, correlations between the neuroticism and the Self-compassion Scale and its subscales were revealed. Structural equation modeling was involved to gain more complex insight in the researched area.