Despite the increasing recognition of positive life skills interventions to enhance the psychological and physical well-being of individuals, there is an absence of a valid and reliable Arabic measure of Life Skills (LS) amongst youth. This study aims at validating an Arabic Life Skills Scale (A-LSS) to assess the effectiveness of life skills interventions. A randomly selected sample of 1200 university students in Lebanon participated in this study. The exploratory-to-confirmatory factor analyses strategy was followed to examine the factor structure of the LSS. For that purpose, the sample was divided into two subsamples. The exploratory factor analysis results showed a nine-factor solution, with a strong reliability for the total scale (McDonald's omega [omega] = 0.95) and all subscales ([omega] ranging from 0.68 to 0.91). The confirmatory analysis results showed excellent fit indices (CFI = 0.98, TLI = 0.97, GFI = 0.95; RMSEA = 0.031 [90% CI 0.028-0.034] and SRMR = 0.061), after correlating residuals of items 53 and 54. Measurement invariance across genders was verified at the configural, scalar and metric levels. The A-LSS showed high reliability and validity of LS among Lebanese university students. This scale can be used as a valid tool to measure effectiveness of life skills health promotion interventions.