A replication study was conducted to determine the effectiveness of an instructional treatment based on self-efficacy theory when used with novice Arduino microcontroller users. Students (n = 32) in an introductory university agricultural systems technology course participated in a lesson on Arduino microcontrollers, circuit breadboarding, and Arduino programming which included four hands-on practice tasks, designed to provide students with positive mastery, vicarious and social persuasion experiences. Next, students completed a laboratory activity and were provided additional opportunities for mastery, vicarious, and social persuasion experiences. The one-group pretest-posttest design indicated the instructional treatment had significant (p < 0.001) and large effects in increasing students' interest in Arduino, breadboarding self-efficacy, programming self-efficacy, and Arduino knowledge. These findings were consistent with the original study and provided additional evidence for self-efficacy theory as an effective model for developing instruction for novice Arduino users. Students' written comments provided additional insight concerning the instructional treatment.