Although an abundance of research has discussed the importance of Universal Design for Learning (UDL), there is little research on how UDL may be used in syllabus construction. The authors developed and validated the UDL Syllabus Scale (UDLSS), which evaluates higher education instructors' ability to design a syllabus using UDL principles. Over 250 higher education instructors completed the UDLSS, the Multicultural Competency Teaching Inventory, the Learner-Centered Syllabus Scale, and the Teacher Self-Efficacy Scale. Results indicate that the UDLSS produced two factors, UDL Engagement and Representation and UDL Action and Expression, and that it demonstrates strong internal reliability and concurrent validity.