For preservice teachers, inclusive education practices are daunting, highlighting concerns around confidence, individualisation, and student behaviour. To explore this issue further, this study examined the perceptions of preservice Health and Physical Education (HPE) teachers on inclusion using a sequential, explanatory mixed-methods research design. Preservice HPE teachers (n = 44) completed a compulsory course on inclusion and a 10-week school internship. Sentiments, attitudes, and concerns were examined using the Sentiments, Attitudes and Concerns about Inclusive Education-Revised Scale (SACIE-R) and reported a Total Scale Score (TSS) and respective Sub-Scale Scores (SSS) for Sentiments, Attitudes and Concerns across three time points. Linear mixed models showed a significant improvement in TSS (p = 0.005) over time from course participation to internship. In addition, the SSS for Concerns was significant over time (p < 0.001) resulting in reduced Concerns about inclusion as time progressed. Qualitatively, six students participated in semi-structured interviews that examined views of inclusive education practices. Findings suggest an improvement in attitudes towards inclusive practices through participation in specific courses that provide direct opportunities for preservice teachers to practice inclusion. Implications for preservice teacher education programmes include the importance of direct experience with and without the pressure of school environments.