This study compared reported chemistry anxiety in an undergraduate chemistry course between online and classroom students. Results indicated slightly higher levels of anxiety for online students although findings were not statistically significant. However, activities resulting in high anxiety for both groups were identified. Gender was identified as a moderating variable with significantly more women than men indicating a high anxiety level when entering the lab and getting chemicals on their hands. Gender should be explored as a variable for anxiety and confidence when working with chemicals. Curriculum designers can also use the survey and methodology to evaluate their programs.