Historically, traditional laboratory experiments have been given a central and distinctive role in science education. However, virtual laboratories have received considerable attention over the past several years in different areas of knowledge. The main aim of this study is to analyse the impact of the use of both traditional and virtual laboratories in Basic Science Subjects (BSS) in Health Sciences university degrees. For this reason, we have described and analysed different variables such as level of satisfaction, increase in perceived motivation and increase in perceived academic performance. Throughout the academic course the pupils who took part in our study carried out laboratory experiments both traditionally and virtually in different BSS, and they completed a questionnaire to assess their satisfaction and the impact of both approaches at the end of the course. The results point to statistically significant differences in favour of traditional laboratories in all the studied variables, together with some differences between male and female students. In general, students showed more positive attitudes towards traditional experiments. These results emphasize the importance of carrying out hands-on experiments to boost student motivation and perceived performance.