Human ageing and age-associated diseases are among the greatest challenges and financial burdens faced by developed countries. Unfortunately, undergraduate students often do not experience any laboratory training in ageing research in the current laboratory curriculum of majors in biology, biotechnology, or medicine. In this study, we developed and implemented a 12-week laboratory course for biotechnology undergraduates in which "Drosophila melanogaster" is used as the model organism to scaffold the understanding of the basic concept and process of ageing research. Students worked in teams of three to conduct lifespan analyses based on the use of selected dietary supplements on "Drosophila melanogaster." By integrating abstract knowledge and practice in the process of scientific research, students gained hands-on experience in lab techniques while learning about the principle and process of ageing research. The learning outcomes demonstrated that students substantially enhanced their understanding of how lifespan assays could be used to evaluate whether a certain dietary supplement has an anti-ageing effect. Overall, the course was very effective for helping biotechnology undergraduates learn the theory and application of basic ageing research, while also yielding positive effects in developing confidence and scientific skills for future work in research.