Purpose: The purpose of this research paper is to adapt and validate a useful instrument to diagnose the knowledge, attitudes, behaviours and intention to participate (KABIP) towards Sustainable Development Goals (SDGs) in higher education institutions (HEIs) from the public administration in developing countries. Design/methodology/approach: The study was carried out using the Delphi technique, exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). For the Delphi method, 30 experts participated in three rounds. For the EFA and CFA, a sample of 790 people was selected. The instrument items were extracted from relevant scales on the topic. Findings: The reliability and internal consistency of the instrument were tested and confirmed. Therefore, this study gives a scientific tool to measure the KABIP towards SDGs in different sectors of the public administration, and it can be used in developing countries. Originality/value: The new questionnaire with 66 items and four dimensions can be used to diagnose KABIP towards SDGs at HEIs in developing countries. It has a very important implications because, although attention for SDGs around the world is increasing, only a limited number of valid scientific instrument that measure aspects related to it is available. Furthermore, the knowledge, attitudes, behaviour and intention to participate scale can facilitate the creation of new strategies regarding SDGs in developing countries.