Objective: To examine the effects of a 15-week, conceptually based university health/wellness course on moderate-to-vigorous physical activity (MVPA) levels, psychosocial factors, and health-related fitness knowledge (HRFK). Participants: 125 undergraduates enrolled during spring 2016 semester. Methods: Participants completed pre- and post-course surveys with questions on MVPA levels, exercise self-efficacy (SE), exercise motivation, and HRFK. Wilcoxon rank sum tests examined changes in SE, motivation, and HFRK. A multiple regression analysis examined associations among HRFK, psychosocial factors, and MVPA. Results: HRFK and MVPA increased from baseline to post-course assessment (p<0.01). An increase in SE was associated with an increase in MVPA (p < 0.0001). Conclusions: Enrollment in a conceptually based health/wellness course may increase MVPA and HRFK in college students. Although the increase in SE post-course was not significant, it was associated with an increase in MVPA. These results support adoption of health/wellness programming to attenuate decreases in MVPA typically observed in college students.