Women and men are capable of effectively discharging school leadership roles. However, in Ghana, people are socialised to expect females to lead girls' schools and vice versa. Appointing a female or male to lead a single sex school that is opposite to their gender elicits gender stereotyping, protests and exclusion. While researchers have explored these dynamics in co-educational basic schools, they have not extended them to single sex schools. This case study investigated how the leadership practice of a male-led girls' school in the Central Region of Ghana has challenged gendered walls. The study engaged 23 multiple stakeholders and gathered data through semi-structured interviews, observations and document analysis. Intra- and cross-case analyses of data revealed that loyalty to gender walls in single sex schools reflects the desire to respect and maintain the tradition of female headteachers in girls' schools and male headteachers in boys' schools. Overall, Ghana is still a traditional and patriarchal society that is characterised by gender inequity in leadership spaces. However, men and women who exhibit professional integrity and leadership competence could convert critics to supporters in a range of school settings. The Ghana Education Service should provide leadership preparation for practitioners to improve their leadership competence.