The transition of our large ([approximately]300 student) introductory soil science course to the online setting created several challenges, including engaging first- and second-year students, providing meaningful hands-on learning activities, and setting up online exams. The objective of this paper is to describe the development and use of question banks for online exams and other forms of assessment in an introductory soil science course. Use of question banks for the development of the online exams, especially in large classes, may be advantageous due to time saving features such as automatic marking of quiz style questions, the ability to easily setup practice exams, and question randomization to reduce the risk of misconduct. Instructors should keep in mind that questions in the question banks should be aligned with course learning outcomes, and organized by theme and level of difficulty. Through a case study of this course, we hope to provide lessons learned which may be applicable to other large introductory science courses.