The e-learning platform provides a new teaching-learning channel in which instructors provide information to learners irrespective of method used to access the platform. The purpose of this study is to examine instructors' acceptance of e-learning in Nigerian universities. The study adopted a quantitative approach, with a total of 299 questionnaires collected from instructors. The result was analyzed using PLS-SEM. The study results indicate the factors affecting instructors' adoption of e-learning platforms and the subsequent impact on instructors' behavioral intention, and consequently, an impact on the acceptance of the e-learning platform in Nigeria. Based on the study, self-efficacy, educational quality, and ease of use, perceive usefulness, and behavioral intention were found to be predictors for instructors' acceptance of the e-learning platform in Nigeria. Also, the results drawn from the study revealed that there are individual challenges that come from the use of the e-learning platform. We discussed both theoretical and educational implications.