Objective: Access to naloxone is a priority for reducing opioid overdose deaths. Although participants who receive naloxone training are able to successfully administer it, the impact of training on community member knowledge and attitudes has not been explored. Participants: A total of 105 participants 18 years and older at a medium-sized university completed assessments between August and December 2019. Methods: Participants were scheduled to complete a standardized 30-minute naloxone training as developed by the research team. Assessments were taken at baseline, immediately following, and three-months following education using the Opioid Overdose Knowledge Scale (OOKS) and Opioid Overdose Attitudes Scale (OOAS). Results: The primary endpoint of change from baseline to immediate post-education for total OOKS and OOAS median scores significantly improved (p < 0.001) by 31 and 34 points, respectively. Conclusions: Naloxone education provides an opportunity to improve community attitudes and reduce stigma over time.