Development of 21st century skills and competencies in teaching and learning remains a key strategic imperative. Coherent development of skills and competencies requires adoption of innovative pedagogical strategies. Technology integration can be harnessed to foster effective teaching and learning. The study examined the role of technology integration in the development of 21st century skills and competencies in Life Sciences teaching and learning. The empirical investigation adopted an explanatory sequential mixed method design and involved 15 purposively selected teachers from five South African suburban schools. The study is underpinned by social constructivism as the underlying theoretical framework. Quantitative data was collected through the administration of a survey questionnaire with the participants while qualitative data was collected through semi-structured interviews. Technology integration was perceived to promote the acquisition of 21st century skills and competencies in Life Sciences teaching and learning. In particular, the teachers indicated that technology integration facilitates the development of skills such as communication, critical thinking, collaboration, problem solving and computational thinking. In addition, technology integration was largely perceived to create exciting teaching and learning environment which fosters the enhancement of academic achievement and motivation of learners. Theoretical implications for technology-enhanced teaching and learning are discussed.