Distance education has become an important opportunity for teachers who do not use technology or use it little in learning environments to use ICT tools such as graphic tablets, interactive content and dynamic geometry software by requiring them to perform their courses with technological tools. In this regard, the present study aims to examine the technology acceptance and TPACK competencies of mathematics teachers in the distance education process that has been put into practice due to the impact of the pandemic on a global scale. In the study, exploratory correlational and causal-comparative research models were used. The sample of the study consists of 223 teachers selected from the mathematics teachers who perform mathematics education practices through distance education with criterion sampling, one of the purposive sampling methods. The personal information form, Technology Acceptance Scale for Teachers and TPACK Scale were used as data collection tools. In order to examine whether the technology acceptance scores and TPACK levels differed according to various variables, the independent t-test and the analysis of variance (one-way ANOVA) were applied.