The goal of this study is to assess the referral rate accuracy of photoscreening versus the chart methodology in identifying preschool children at risk of amblyopia and amblyogenic refractive error. Vision screenings using the plusoptiX S12 and the LEA chart were performed on 127 children, aged 3-5 years old. Comprehensive eye exams were performed after screenings. The sensitivity and specificity of the plusoptiX S12 were 80.3% and 92.1% and the LEA chart were 43.6% and 94.8%, respectively. The sensitivity of the plusoptiX S12 is significantly higher than the LEA (p value: <0.001). After eye exams, 82.9% were correctly passed by the plusoptiX S12 and 64% were correctly passed by the LEA chart (p value: 0.009). Objective photoscreening is significantly more accurate in identifying preschool children at risk of developing amblyopia and should be considered best practice. The chart methodology provides an inaccurate report on a preschool child's amblyopic risk.