This study examines the creation and implementation of a video-based assessment of teacher knowledge of effective teaching called the Video Assessment of Teacher Knowledge (VATK). The VATK was developed with the intention of creating an easily administered, standardised assessment that can potentially provide information on teacher candidate knowledge of teaching at important points during their training programmes and into the teaching field. The theoretical framework upon which the VATK was developed and the process for item creation are described. Classical test theory and item response theory analysis were conducted to determine the measure's potential for use in future studies. Analyses indicate that after some questions are removed, the measure is sensitive to differences in teachers' pedagogical knowledge.