The purpose of this study is to examine the life satisfaction and automatic negative thoughts perception of physical education, art and music pre-service teachers. Students (n=387) who training in physical education and sport, art work and music departments participated in this study. Results showed that physical education teacher candidates' life satisfaction scores were significantly higher than those of art and music teacher candidates. On the other hand, the automatic negative thought perceptions of the art and music pre-service teachers were found to be higher than the physical education teacher candidates. Life satisfaction levels of the physical education pre-service teachers are found to be high, negative thought levels were low. Current findings emphasize the importance of participating in sports activities in order to increase the life satisfaction levels of pre-service teachers and decrease their negative automatic thinking levels.