This survey study discusses the perception of work-life balance among married female academic librarians in university libraries in South-East Nigeria. Purposive sampling technique was used to select 90 married female academic librarians from a population of 150 female academic librarians working in the university libraries. The study examines the perception of family and organizational factors of work-life balance among the respondents. The results reveal that the married female academic librarians have a positive perception of family and organizational factors of work-life balance; however, perception of the organizational factor has a slightly higher mean score than the family factor. The study highlights strategies that could enhance work-life balance among the respondents and recommends the implementation of practices and policies such as flexible work schedule, cancellation of overtime and shift duties, and better remuneration to improve work-life balance among academic librarians.