With the growing popularity of eSport games, eSport-related issues have gradually gained attention and discussion in academic research. However, the positive benefits (values) brought by playing eSport have not received too much attention in current research. Therefore, after reviewing related research in the past, this study proposed that eSport has the 5 Cs educational Value of cultivating the cooperative attitude, communication skills, critical thinking, self-confidence and continuous improvement attitude based on the three-domain model (TDM) of cognitive, affective and psychomotor, and developed an eSport educational values scale. In this study, a conceptual sampling method was adopted and players with eSport experience were invited to fill out the questionnaire. A total of 316 participants filled out the questionnaire, 51 invalid samples were deleted, the number of effective participants was 265, and the effective recovery rate was 83.9%. Then SPSS 23.0 and AMOS 20.0 were used to analyze the reliability and validity of the scale, and the verification results show the scale developed by this study has good reliability and validity. In addition, in this study, it was also found that the participants had a positive view (M > 3.9) on the 5 Cs educational value of the MOBA type eSport, which shows that eSport is not only a casual game, moderate playing this game can also bring educational significance to players.