Biology is a complex science that includes different subjects. As a result, misconceptions, confusion and misleading are common problems in biology education. In this study, it was aimed to determine the learning of pre-service science teachers about food web. The research, using the case study, was conducted in 2019-2020. The study group of the research consists of 76 pre-service science teachers. The structured interview form and focus group interview were used to collect the data. As a result of the research, it was determined that pre-service science teachers have misconceptions, confusion and misleading about food web. When the reasons for the pre-service science teachers' misconceptions, confusion and misleading about food web were examined, it was found out that teachers, textbooks, supplementary textbooks and individual errors were the reasons. Pre-service science teachers should be supported with scientific training in order to learn the food web correctly. In addition, international scientific books should be used in the preparation of textbooks on food web.