During the COVID-19 epidemic, online teaching was widely used at Nanjing Medical University. Based on the practice, student evaluation surveys were used as the main assessment to analyze the effect of online teaching. Eventually, 826 undergraduates at Nanjing Medical University participated in the questionnaire survey. This study described the present situation of online studying among medical students, and some suggestions were put forward. The survey showed that online education could not replace face-to-face learning, but it presented great potential for application in teaching fields.