Low physical activity and sedentary lifestyle have been contributing to overweight/obesity in children worldwide. In response to this, school health and physical education (HPE) programmes have been introduced to assist children to adopt and maintain healthy lifestyles. Teacher education colleges have been introducing training programmes to prepare teachers. Paro College of Education (PCE), Bhutan, is one such college where the HPE training programme was introduced in 2003. This paper reports on the impact of the HPE programme on the preservice teachers of PCE in relation to their personal and professional competencies. The participants were the final and second-year Bachelor of Education (primary) pre-service student teachers and Diploma in Physical Education and Sports Coaching students. Results revealed that the HPE programme had a significant impact on pre-service teachers' personal and professional competencies. Male participants were highly motivated to teach HPE as well as participating regularly in physical activities compared to their female counterparts. Further, the study revealed differences in personal and professional competencies, pedagogical knowledge, and self-efficacy beliefs amongst the pre-service teachers. Implications for the teacher training programme and recommendations for future research are presented.