Organizational culture is crucial for all living institutional structures and has become a critical research topic. Although different variables have been investigated in the literature as predictors of organizational culture, to our knowledge, the role of social values and life skills as mediators hasn't been investigated before. This study, therefore, aims to investigate the role of social values and life skills as mediators of organizational culture. The hypotheses of the research were tested in the context of this research aim. The present study, which is in the relational survey model, included 370 participants consisting of teachers and school leaders working in 22 different educational institutions. The findings showed that teachers and school leaders had high levels of organizational culture, social values, and life skills. The relationship of organizational culture with social values and life skills is meaningful and high. Also, the findings indicate a partial effect on mediating organizational culture between social values and life skills.