The aim of the research was to investigate the relation between middle school students' perceptions of school climate and school motivation, whether school climate perceptions have a significant power on school motivation as well as to see if those perceptions and motivation differ significantly depending on gender, grade level, and school location variables. The sample of the study consisted of 674 middle school students studying in two state schools, which were located in the central district and village in Kosk province in Aydin in Turkey. Data were collected by using School Climate Perceptions Scale and School Motivation Scale with three questioned personal information form. Results showed that there were significant medium-level correlations between school climate perceptions and school motivation of middle school students. While grade-level variables had significant differences in both school climate perceptions and school motivation of middle school students, the location did not create a difference. On the other hand, gender had a significant difference only with school motivation. Finally, the model tested student-teacher relation significantly predicted 18% of the school motivations of middle school students.