Objective: To assess the characteristics of campus populations who were opposed to a vape-free campus policy and examine factors associated with opposition to a vape-free campus policy among those who indicated support for a tobacco-free policy. Participants: Faculty, staff, and students (N=2210) in a Midwestern university participated. Methods: Individuals were invited to a campus-wide online survey about the tobacco-free policy on campus in spring 2018. Pearson's X[subscript 2], t-test, and binary logistic regression were used for analysis. Results: Age, gender, current tobacco use, perceived harmfulness of e-cigarettes, and perceived harmfulness of secondhand smoke were significantly associated with opposition to the vape-free campus policy. Conclusions: Our data highlight the importance of various demographic factors that are associated with opposition to the vape-free policy. The current field needs to use informative approaches to improve knowledge of overall tobacco in health campaigns and public health programs on campus and within community outreach programs.