A practicum is beneficial in science learning to provide a concrete manifestation of a topic. This study aimed at creating a simulation-based interactive learning multimedia product to assist students' practicum. The product was developed for the topic of cell metabolism process focusing on the energy in living systems. The study employed the Research and Development referring to the Analysis, Design, Development, Implementation, and Evaluation model. During the development process, both product testing and users' response testing were carried out. To reveal the product impact, students' results of the pre-test and post-test were analyzed. The product was developed by using Macromedia Flash 8. The results of Alpha and Beta analysis showed that the results are 'feasible' and 'very feasible' for the product. Furthermore, based on the average value of 39.2 for the initial test and 82.5 for the final test it was obtained an Ngain value of 0.712 with the category of "High". Therefore, it proved that the developed interactive learning multimedia is effective in increasing students' analytical skills for the Science Learning materials in junior high schools.