The present study aimed to examine and validate a rubric for translation quality assessment using Rasch analysis. To this end, the researchers interviewed 20 expert translation instructors to identify the factors they consider important for assessing the quality of students' translation. Based on the specific commonalities found throughout the interviews, a 23-item assessment rubric was constructed on a four-point Likert scale. More specifically, this study used the Rasch rating scale model for polytomous data to investigate the psychometric properties of the rating scale in terms of dimensionality, reliability, use of response category, and sample appropriateness. Then, a translation exam was administered to 60 translation students at the BA level in Iranian universities. Following this, the rubric was employed to assess the quality of students' translation. The results revealed that the Rasch model fits the data well. Thus, the findings of the study indicated that the rubric is potentially valid and useful, and can be used as a measure of translation quality assessment in the Iranian context.