There is an identified need in the research literature for the design, implementation, and evaluation of a conceptual framework for creating contextual, interactive mobile learning. This article details how the conceptual framework was implemented and tested in an online learning course for physicians, nurses, and healthcare professionals at medical organizations across Ontario. The conceptual framework and evaluation instruments were revised based on identified best practices and feedback from study participants. This provided a practical, evidence-based tool for informing the effective design of mobile learning. Results indicated that the design of the framework to include context-specific content, guided participation delivery, flexible and intuitive usability, formal online and informal mobile structure, and access to communities of practice all resulted in practical, applicable learning outcomes and a high degree of learner satisfaction.