This article analyses the first women to hold teaching and research positions in Spanish universities during the first third of the twentieth century. It traces the paths of the pioneers who used their intellectual capacity to broaden the scope for their working lives. Legal changes introduced in 1910 made it possible for women with a university degree to work as professors in the university or in secondary schools, and in libraries, museums or archives. The writer Emilia Pardo Bazán, was the first woman to be appointed as a lecturer for her literary merits. She taught on a PhD course at the University of Madrid, and was followed by other female lecturers, at the Faculties of Philosophy and Letters, Science, Pharmacy and Law; in this order. The paper recalls these first female lecturers, the posts they occupied, the situations they encountered, and their overall trajectories. All began to work in the midst of a strong androcentric culture. Very few of those who started as lecturers before 1936 -- the outbreak of the Spanish Civil War -- continued later at university on a permanent basis. The majority chose other types of employment which offered greater stability in less time.