This study investigated the influence of spontaneous focusing on numerosity (SFON) tendency on the performance and estimation patterns on number line estimation (NLE). To examine this question, 147 preschoolers (3.51-4.52 years) completed two types of SFON task (referenced SFON task and non-referenced SFON task) and three conditions of NLE (non-symbolic area, number-to-position, and position-to-number). The participants' SFON tendencies were classified as high, average, and poor using latent profile analysis. Children with high SFON tendency performed better and preferred to use more advanced strategy than their poor-SFON peers on NLE tasks. The proportion of children with irregular NLE pattern increased as SFON tendency decreased. These findings contribute to our understanding of the relationship between performance patterns of different NLEs and SFON tendencies for preschool children.