The Michigan State University Motor Performance Study (MPS) was initiated in 1967 and lasted 32 years. Three central components existed: 1) Educational component, in which students experienced applied work with youth; 2) Instructional component, whereby enrolled youth received sport-specific skill instruction; and 3) Research component. The primary goals of the research component of the MPS were to examine: 1) the changes over time that occur in the physical growth, biological maturity, and motor skill acquisition of children and youth; 2) the processes involved in the attainment of basic and complex motor skills; and 3) the influence of changes in the learners' environment on their rates of motor skill acquisition. Several growth and maturation, motor competence, and physical performance/fitness variables were collected, and a follow-up study examined participants' adult physical activity, sport participation, and health outcomes. This manuscript describes methods used in the research component of the MPS and the follow-up.