Information literacy is an essential skill for biologists; however, most biology curricula do not intentionally integrate information literacy into classroom and laboratory exercises. There is evidence that developing information literacy skills in undergraduates improves their research skills, writing, and GPAs. Our objective was to integrate information literacy skills into a first semester introductory biology laboratory with a multi-week, inquiry-based module that leverages primary literature. Here we describe the module, which challenges students to develop and test a hypothesis related to parental care behaviour in birds. Students form hypotheses based on literature searching done during librarian-led information literacy sessions, produce an annotated bibliography, collect and analyse video data of barn swallows feeding their offspring, and present their findings. Analysis of students' annotated bibliographies indicates that 83% of the referenced papers were appropriate for developing their specific hypotheses. The key elements of a successful information literacy training plan include faculty-librarian collaboration, multiple classroom or laboratory sessions that introduce or utilize information literacy, and relevance of the information literacy training to an assignment. By introducing information literacy early in biology curricula, departments can develop tiered information literacy plans that incorporate opportunities for students to use and refine these skills throughout their studies.