In recent years the delivery of practical laboratories classes has been subjected to debate, with regards to pedagogical efficiency. Our aim was to develop a pre-laboratory online learning resource that enhances the preparedness and performance within laboratory classes. A study was conducted using second year Pharmacy students, in which the class was given access to an online learning resource that included visual information, quizzes, safety and theory and this was compared to the laboratory script as a preparative resource for the class. Less advice was requested by students and reduced error in practical skills was observed when the online learning resource was used by students, when compared to the laboratory script. Students felt they were able to use the apparatus without the requirement of support, and thus were more confident in the laboratory class. This online resource has the potential to overcome the pedagogical limitations associated with traditional delivery of laboratory classes.