This study aimed at determining the factors that influence teacher acceptance of the application of the e-Report Junior High School application with the Human Organization Technology (HOT) Fit Model approach. The population in this study used all teacher in SMP Laboratorium Undiksha Singaraja and SMP 2 Singaraja amounted to 150 respondents. Sampling using proportional random sampling technique. The sample in this study amounted to 67 respondents. Primary data collection was obtained based on questionnaires to respondents. The Instrument of questionnaires was validited by content judges to get validity and reliable instrument. Data were analyzed using Structural Equation Model-Partial Least Square (SEM-PLS) with SmartPLS 3.0 software. The results of this study indicated that (1) System quality did not affect system usage, information quality did not affect system usage, and service quality had a positive effect on system usage of 36.7%. (2) The quality of the system did not affect user satisfaction, the quality of information had a positive effect on user satisfaction by 50.6%, and service quality did not affect user satisfaction. (3) User satisfaction had a positive effect on system usage by 15.4%. (4) Organizational structure had a positive effect on system usage by 25.2%. (5) The use of the system did not affect the net benefit, user satisfaction had a positive effect on the net benefit of 62.1%, and the organizational structure did not affect the net benefit.