Universities assess their academic learning environment to improve students' learning. Students' experience in e-learning environment is different from face-to-face educational environment. So, in this study a specific valid and reliable instrument was devised for assessing perception of e-students from educational environment, that is, educational atmosphere. Firstly, we devised the primary instrument based on factors constituting educational atmosphere. Then Instrument's content and construct validity were assessed. Also, Cronbach's alpha and test-retest were used for studying the internal consistency and reliability of the instrument respectively. The final instrument named 'e-learning educational atmosphere measure' (EEAM) consisted of 40 items covering six factors, including programme effectiveness, teaching quality, ethics and professionalism, learner support, safety and convenience, and awareness of the rules, which accounted for 68.53% of variances. Content validity ratio was more than 0.51 and content validity index score of all questions was above 0.81. Test-retest reliability was 0.85 (p = 0.001) and Cronbach's alpha was 0.943. Assessing educational atmosphere in e-learning settings by EEAM could provide managers and investors with useful information to settle an effective education system by prioritising the necessary changes.