The purpose of this study was to investigate the pre-service teachers' attitudes towards teaching profession and explore the effects of the certain variables (gender, GPA, age, department, motives for selecting teaching profession, and the level of mother and father education) on their attitudes. The participants of the study consisted of 344 undergraduate students, (282 females and 62 males) from five different departments at the faculty of education at Middle East Technical University during the fall semester of the 2015-2016 educational year. As a means of data collection, the "Attitude Measurement Scale for Teaching Profession" developed by Üstüner (2006) was applied. The scale was a one-dimensional 5-Likert type ranging from "strongly disagree (1)" to "strongly agree (5)" with 34 items. For the analysis of the data, Independent-Samples t-test and One-Way-ANOVA were used. According to the findings obtained from the study, while the attitudes of pre-service teachers towards the teaching profession showed a significant difference regarding gender, GPA, department, and motives for selecting the teaching profession, it did not show any significant difference for age and the level of mother and father education.