Purpose: In Australian Football (AF), the ability to proficiently kick the ball is a critical skill and has been shown to be advantageous to a team's successful performance; however, a valid and reliable match referenced kicking assessment remains absent. Therefore, the aim of this study was to develop a valid and reliable AF kicking proficiency assessment comparative to match play kicking performance. Method: Youth male Australian Football players (n = 251) from different stages within the AF talent pathway were recruited. The developed AFFB-DKA (Australian Football Field-Based--Dynamic Kicking Assessment) considered particular constraints of match play kicking demands such as kick type, distance, delivery, and locomotion of the player receiving the ball. In total, 14 kicks were completed during the test. Validity (i.e., content, logical and construct) and reliability (i.e., test re-test) were assessed. Results: Findings indicate the kicking test can distinguish across and between age (i.e., U14; U16; U18) and skill groups (i.e., club; sub-elite; elite). The timeframe between U14 and U16 was identified as a potential key period where kicking skill acquisition may be most impressionable; however, further research is recommended to support this. Conclusion: The developed AFFB-DKA is the first Australian Football specific kicking assessment to consider and apply match play kicking constraints to make a more representative, valid and reliable assessment.