The aim of this study was to investigate the effects of agility training on reaction time in fencers. Accordingly, 48 athletes (24 females and 24 males) actively involved in fencing participated in the study. Fencers were divided into two groups as 24 fencers in the Agility Training Group (ATG: 12F, 12M) (aged 11.95 years, sports age 2.54 years, height 159 cm, body weight 48.08 kg and body mass index 18.81 kg/m[superscript 2]) and Conventional Training Group (CTG: 12F, 12M) (aged 12.12 years, sports age 2.20 years, height 156.54 cm, body weight 46.25 kg and body mass index 18.81 kg/m[superscript 2]). There was a statistically significant difference between the pre-test and post-test values in agility, vertical jump, simple reaction time and multiple reaction time tests in the ATG (p<0.05). There was a statistically significant difference between the pre-test and post-test values in agility and vertical jump tests in the CTG (p<0.05). There was a statistically significant difference in the pre-tests between the groups in the simple reaction time test (p<0.05). There was a statistically significant difference in post-tests between the groups in the agility, simple reaction time and multiple reaction time test values (p<0.05). Based on the results of the study, it was found that simple and multiple reaction time could be positively affected by active-reactive agility training applications.