This study aimed to examine relationships among the confidence of pre-service physical educators in teaching individuals with disabilities (IWDs), their previous experiences, and the quality of experiences and to test if the relationship between the quality of previous experiences and confidence in working with IWDs is mediated by the previous experiences. A total of 150 pre-service teachers completed a questionnaire. Significant relationships were found between previous experiences and the quality of such experiences (r = 0.70, p < 0.001), previous experiences and confidence (r = 0.50, p < 0.001) and the quality of previous experiences and confidence (r = 0.35, p < 0.001). The regression analysis indicated that the quality of previous experiences was a significant predictor of confidence, [beta] = 0.35, F(1, 148) = 20.19, p < 0.001. The quality of previous experiences was related to previous experiences, [beta] = 0.70, F(1, 148) = 142.76, p < 0.001. Previous experiences was associated with confidence, [beta] = 0.50, F(1, 148) = 50.18, p < 0.001. The results of the mediation analysis confirmed the mediating role of previous experiences in the relationship between the quality of previous experiences and confidence, [beta] = 0.24 (CI = 0.15 to 0.33). After controlling for previous experiences, the direct effects of the quality of previous experiences on confidence became nonsignificant, [beta] = -0.01, t (147) = -0.12, p = 0.904. Finally, the results of the bootstrapping test showed that the mediation model was significant, Z = 4.69, p < 0.001, [kappa][superscript 2] = 0.24.