Extension program assistants' turnover intention is a key indicator of human resource development practice and overall effectiveness of organizational leadership. We used a survey design with four instruments to investigate the factors that influenced 149 Ohio State University Extension program assistants' turnover intention. The data showed significant associations between employees' age, years of service, job satisfaction, supervisor satisfaction, organizational commitment, and turnover intention. Organizational commitment mediated the relationship between job satisfaction and turnover intention. The research concludes with recommendations that may help to decrease extension program assistants' turnover intention.