Objective: To examine the use of transportation networking companies (TNCs) (eg, Uber) among substance-using students in rural and urban college settings. Participants: Students at two large state universities were randomly selected and screened for substance use. Participants reported use of TNCs generally and after substance use and whether TNC use was on or near campus or in other environments. Methods: Data were evaluated using chi-square test, t-tests, and Fisher's exact tests. Results: Most (85%) participants (n = 99, 61% response rate) had used a TNC. Among students who used TNCs on/near campus, 98% of rural students used them after substance use compared to 85% of urban students (p = 0.037). We did not detect differences in TNC use by gender or age. Conclusions: Results indicate that TNC use is common after college student substance use and may play a particularly important role in preventing impaired driving for rural campuses where existing transportation options are limited.